Screening of potato germplasm against RNA viruses and their identification through ELISA

Abstract

Fifteen potato advance lines were screened against Potato Leaf Roll Virus (PLRV), Potato Virus X (PVX), Potato Virus Y (PVY) under favorable natural field conditions and in vitro by using double antibody sandwich ELISA (DAS- ELISA) against six potato viruses viz; PLRV, PVX, PVY, Potato Virus S (PVS), Potato Virus A (PVA) and Potato Virus M (PVM) at Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan during 2012-2013. On the basis of symptomatology, only two lines FD70-1 (PRI RED), SL15-26 were found free of symptoms to PLRV. No one genotype was found to be highly resistant against PVX and PVY. Environmental factors had a great influence on disease severity. In ELISA screening no one advance line was highly resistance against six potato viruses. Three lines namely viz; FD70-1 (PRI RED), SL15-24 and SL15-26 were found resistant, five moderately susceptible and seven susceptible in case of PLRV. In PVY, two advance lines i.e. FD70-1 (PRI RED) and SL14-05 were found resistant. In PVX, only one line SL15-26 was found to be resistant. In PVA, four resistant advance lines, eight moderately susceptible and three were found as susceptible. In PVS, only one resistant genotype, six moderately susceptible and eight were found as susceptible. Likewise in PVM there is no genotype as a resistant, eight moderately susceptible and seven were found as susceptible. The resistance germplasm of potato screened under field conditions and by ELISA of different potato viruses may be used to produce the virus free germplasm in the next breeding programme.